The Journal of Agricultural Science Nr. 1(01), pp. 3-9
ХАА-н Шинжлэх Ухаан сэтгүүл №1(01), хуудас 3-9
ÃÀÕÀÉÍ ÑÀËÌÎÍÅË˨ÇÛà ÎÍÎØËÎÕ, ÑÝÐÃÈÉËÝÕ ªÂªÐÌªÖ ÀÐÃÀ
Á. Íàðàíãýðýë, À.¨íäîíäîðæ
Ìàë ýìíýëãèéí õ¿ðýýëýíãèéí õàëäâàðò ºâ÷èí äàðõëàà ñóäëàëûí ëàáîðàòîðè
bnaran2001@yahoo.com
Õóðààíãóé
Ìàíàé óëñ çàõ çýýëèéí ýäèéí çàñàãò øèëæñýíýýñ õîéø ãàõàéí æèæèã äóíä àæ àõóéíóóä îëíîîð áàéãóóëàãäàæ îäîîãîîð 32800 òîëãîé ãàõàé ¿ðæ¿¿ëæ áàéãàà áîëîâ÷ òýäýíä òàâèõ ìàë ýìíýëýã, àðèóí öýâðèéí õÿíàëò õàíãàëòã¿é áàéíà. Òèéìýýñ õ¿íñíèé àþóëã¿é áàéäàë, õ¿í àìûí ýð¿¿ë ìýíäèéã õàìãààëàõ çîðèëãîîð ãàõàéí ñàëìîíåëë¸ç ºâ÷íèéã ñóäëàæ îíîøëîõ, ýì÷ëýõ, óðüä÷èëàí ñýðãèéëýõ çàéëøã¿é øààðäëàãàòàé áàéíà. Ýíý çîðèëãîîð õ¿í àìûí õ¿íñíèé àþóëã¿é áàéäàë áîëîí ãàõàéí àæ àõóé ýðõëýã÷äýä ýäèéí çàñãèéí èõýýõýí õîõèðîë ó÷ðóóëäàã ãàõàéí ñàëìîíåëë¸ç ºâ÷íèéã ººðèéí îðíû íºõöºëä ñóäëàí ¿¿ñãýã÷èéã èëð¿¿ëæ, îíîøëóóð, óðüä÷èëàí ñýðãèéëýõ âàêöèí áýëòãýí ñîðèâ. Ñóäàëãààíû ÿâöàä 508 ýìãýãò ìàòåðèàë, áààñíû äýýæíýýñ 1088 ñàëìîíåëë òºñò ºñãºâºð ÿëãàí àâ÷ òýäíèéãýý öààø íü áàêòåðèîëîãè, áèîõèìè, ìîðôîëîãè, Ïîëèìåðàçèéí Ãèíæèí Óðâàëààð øèíæèëñíèé ä¿íä íóòãèéí 11 îìîã èëð¿¿ëýâ. Ãàõàéíààñ ÿëãàñàí íóòãèéí 11 îìîã íü ºñãºâºðæèëò áóäàãäàõ áàéäàë, õýëáýð ç¿é, áèîõèìèéí èäýâõè çýðãýýðýý Salmonella typhisuis-èéí ñòàíäàðò õýâøèëòýé ä¿éöýæ áàéâ. Ñàëìîíåëëèéí ¿¿ñãýã÷èéã ÿëãàõûí òóëä Ýíäî, Ìàê Êîíêåé, Áðèëëèàíò íîãîîí, Âèñìóò ñóëüôèò çýðýã ñîíãîìîë àãàðóóäûã àøèãëàâ. Îìãèéí áèîõèìèéí øèíæ ÷àíàðûã Ãèññèéí îð÷èí, API-20E ñèñòåì áîëîí í¿¿ðñ óñ, ñàõàðóóäûí äèñê àøèãëàí òîäîðõîéëîâ. Îìãèéí õîðóó ÷àíàð LD-50 (Lethalis dosis 50), DLM (Dosis Lethalis minimium) - èéã öàãààí õóëãàíà õàëäâàðëóóëæ òîãòîîõîä ºíäºð õîðóó ÷àíàðòàé áàéâ. Øèíæèëãýýíèé ä¿íä îìãóóäûã öààøèä ýñðýãòºðºã÷ áîëîí âàêöèí ¿éëäâýðëýëä àøèãëàõ áîëîìæòîéã íü òîãòîîâ. Íóòãèéí îìãóóäààñ 2 òºðëèéí Íààëäóóëàõ Óðâàëûí “Î” áîëîí Íýâ÷èí Òóíàäàñæèõ Óðâàëûí ïîëèñàõàðèäûí “Â” ýñðýãòºðºã÷ áýëòãýí ñîðèõîä ºíäºð òàíüöòàé, ºâºðìºö èäýâõèòýé áàéâ. Íóòãèéí îìãèéí ñàëìîíåëë¸çîîñ ñýðãèéëýõ õîðã¿éæ¿¿ëñýí âàêöèí íü àðèóí, õîðã¿é äàðõëàà òºð¿¿ëýõ ºíäºð èäýâõèòýé, õàìãààëàõ èäýâõè íü 85% áàéëàà. Ñóäàëãààíû ä¿íä 2 òºðëèéí îíîøëîãîîíû ýñðýãòºðºã÷, ýåðýã èéëäýñ, âàêöèí áýëòãýõ òåõíîëîãè òýäãýýðèéã øàëãàõ, õýðýãëýõ çààâàð, ñòàíäàðò á¿õèé òåõíèêèéí 12 áàðèìò áè÷èã áîëîâñðóóëæ ýðõ ç¿éí áàòàëãààæóóëàëò õèéâ.
Ò¿ëõ¿¿ð ¿ã
Íóòãèéí îìîã , Salmonella typhisuis, îíîøëóóð, âàêöèí, ñòàíäàðò
Abstract
In our country during the transition period into market economy were established many swine farms of small and midlle scale, in which are breeding approximately 32800 pigs. But in these farms the veterinary service and sanitation control is unsatisfactory. Therefore, in Mongolia faceing a needs to establish a satisified veterinary control system. The swine salmonellosis causes a big economic damage for pig farmers and food safety aspects of the country.
The purpose of the study were to isolate causative agents and characterize them by cultural, morphological and biochemical properties. And using these isolates had prepared and evaluated antigens for diagnostic tests and inactivated vaccine against swine salmonellosis. Totaly 508 pathological samples were investigated and from them isolated 1088 causative agants lSalmonella spp. From above mentioned agents 11 were identified as a Salmonella typhisuis by cultural, microbiological and biochemical characteristics, which were determined by cultivation on differential mediums such as Endo, Mac Konkey, Brilliant green and Bismuth sulphide agars, Gess medium, disces of carbohydrates and sucrosis and API-20E system, respectively. Finally these isolates were differented by by PCR (Polymerase chain reaction ) as a Salmonella typhisuis. These strains were inoculated subcutaneously into white mice to define it’s virulence and DLM (Dosis Lethalis minimium) and LD50 (Lethalis dosis 50), were determined. Furthermore, we determined the possibilyty to use these field strains for preparation of antigens and inactivated vaccine. ‘O’ antigen for agglutination and agar gel immunodiffusion tests were prepared from these strains. These two different kinds antigens for agglutination and agar gel immunodiffusion tests possess specific quality and high titres.
The model of inactivated vaccine against swine salmonelosis on the control animals showed a characteristics such as a streille, non toxic, and with the high 85 percent protection on animals.
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